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Abstract

The efficacy of a rapid test for detecting PrP(Sc) in central nervous system tissue was evaluated for the post-mortem diagnosis of BSE at different times during the course of the disease. One hundred and six samples of brain, at the level of the medulla oblongata, and spinal cord, derived from the experimental study of the pathogenesis of BSE carried out in Great Britain between 1991 and 1995 were examined. PrP(Sc) was detected in samples from most of the exposed animals killed 32 months or more after they had been exposed to the agent, and before the onset of clinical signs which were first recorded at 35 months. Comparisons with the results of histology, fibril detection, PrP immunohistochemistry and mouse bioassay indicated that the rapid test is at least as sensitive as these conventional confirmatory diagnostic methods and its result can be obtained more quickly.

Additional information

Authors: GRASSI J ET AL, CEA Pharmacology and Immunology Unit, Gif sur Yvette (FR);COMOY E, Bio-Rad Life Science Laboratories, Hercules (US);TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRRM), Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRRM), Geel (BE);HAWKINS S A C, Veterinary Laboratories Agency, Addlestone (GB);WELLS G A H, Veterinary Laboratories Agency, Addlestone (GB);MOYNAGH J, European Commission, DG Health and Consumer Protection, Brussels (BE)
Bibliographic Reference: An article published in: The Veterinary Record, Vol 149, November 10 2001, pp.577-582.
Record Number: 200214545 / Last updated on: 2002-04-04
Category: PUBLICATION
Original language: en
Available languages: en