Community Research and Development Information Service - CORDIS

  • European Commission
  • CORDIS
  • Publications
  • Validation study of an ELISA kit for the quantification of RoundupReady® soy fraction matrices in animal feed ingredients: Report to the 3rd meeting of the European Network of GMO Laboratories, April - October 2001

Abstract

The project involved the collaboration of 21 laboratories and the kit manufacturer, and was coordinated by the Food Products Unit of the JRC Institute for Health and Consumer Protection. The proposed assay, an ELISA kit for the quantification of RoundupReady® soybean fractions in three animal feed ingredient matrices (Defatted Flour, Protein Isolate, Toast meal), showed the following results:

- Repeatability and internal quality performance of the anticipated level (RSD, 11% averaged across all matrices and concentrations).
- Reproducibility lower than expected (RSD (R) ranging from 25% to 45%, depending upon matrix).

The results of the trial clearly demonstrate the issue to be addressed in future assay development, namely the harmonisation of the results from one run to the next through the use of more stable standard curve production.

Additional information

Authors: KAY S, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Food Products Unit, Ispra (IT)
Bibliographic Reference: EUR 20234 EN (2002), pp.28. Euro: Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9843 or +39 033278 9864 Fax: +39 033278 9623 E-mail: jrc-publications-office@ec.europa.eu
Record Number: 200214754 / Last updated on: 2002-05-22
Category: PUBLICATION
Original language: en
Available languages: en