Community Research and Development Information Service - CORDIS

Abstract

The placing on the market of food products derived from genetically modified organisms (GMOs) has raised public concern over their safety and provoked new demands for clear labelling provisions. Regulations 258/1997 and 49/2000 require GMO-containing products to be labelled, and set a limit of 1 % for accidental contamination of non-GM products with GM-material. The need to monitor and verify the presence and the amount of GMOs in agricultural crops and food products has generated a demand for analytical methods capable of detecting, identifying and quantifying either the DNA introduced or the protein(s) expressed. Several laboratories have developed methods based either on DNA detection using the polymerase chain reaction (PCR) technique, or based on protein detection using enzyme linked immunosorbent assays. However, the methods vary in their reliability, robustness and reproducibility, as well as in their applicability to various food matrixes.

In order to obtain comparable results, in which consumers and producers can have confidence, there is an urgent need for reference methods internationally validated. Several analytical protocols have been submitted to ring trial studies at a European and International level. This document summarises the validation status of the tested methods, and discusses their use for enforcement of European food legislation. Section 2 has a summary of the methods for GMO analysis, and section 3 has a discussion on criteria for method validation. This material will provide the framework and scientific guidelines for evaluating the methods included in the ring trials section and into the accompanying database.

Additional information

Authors: KAY S, European Commission, Joint Research Centre, Institute for Health and Consumer Protecton, Ispra (IT);HEINZE P, European Commission, Joint Research Centre, Institute for Health and Consumer Protecton, Ispra (IT);VAN DEN EEDE G, European Commission, Joint Research Centre, Institute for Health and Consumer Protecton, Ispra (IT);BONAFINI L, European Commission, Joint Research Centre, Institute for Health and Consumer Protecton, Ispra (IT)
Bibliographic Reference: EUR 20383 EN (2002), pp.29. Free of charge.
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9843 or +39 033278 9864 Fax: +39 033278 9623 E-mail: jrc-publications-office@ec.europa.eu
Record Number: 200215006 / Last updated on: 2002-07-23
Category: PUBLICATION
Original language: en
Available languages: en
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