Review of GMO detection and quantification techniques
The need to monitor and verify the presence and the amount of GMOs in agricultural crops and food products has generated a demand for analytical methods capable of detecting, identifying and quantifying the DNA introduced or the protein(s) expressed in transgenic plants. Several laboratories have developed such methods, and other analytical technologies that can provide solutions to issues in GM analysis are emerging, e.g. mass spectrometry, chromatography, near infrared spectroscopy, micro fabricated devices, DNA chip technology and nanoscale GMO analysis. Appropriate sampling strategies and methods for sample preparation, as well as GMO detection methods, must be followed by the establishment of validation criteria and performance assessment. Since reference samples are an indispensable part of any analytical protocol, criteria for manufacturing standard reference materials must also be considered. Several methods for different food matrices have been submitted to validation trials. The methods submitted to ring trials varied significantly in their reliability, robustness and reproducibility. More validation studies, especially on the quantitative PCR methods, need to be performed to provide new extraction methods for food matrices and novel GMO constructs.
Bibliographic Reference: EUR 20384 EN (2002), pp.67. Free of charge.
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Record Number: 200215007 / Last updated on: 2002-07-23
Original language: en
Available languages: en