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FP5

Certification of the 17alpha-Trenbolone mass concentration in lyophilised bovine liver reference materials BCR-474 (Trenbolone free) and BCR-475 (Trenbolone positive)

Funded under: FP5-GROWTH

Abstract

Trenbolone (17ß-hydroxy-19-norandrosta-4,9,11-trien-3-one) acetate is an androgenic anabolizing agent used in meat production. Its use is permitted in a number of third countries including the United States of America but is banned in the European Union (Directive 96/22/EC). Trenbolone (TbOH) residues are controlled in urine, faeces, liver, fat and muscle samples. To harmonize the analytical performances within the EU, the "Standards, Measurements and Testing Programme" has funded the development of a series of Certified Reference Materials for veterinary drugs in animal tissues and fluids.

This contribution describes the production and certification of lyophilised bovine liver materials uncontaminated (BCR-474, trenbolone-free, blank material) or ontaminated with trenbolone (TbOH) residues (BCR-475; target value : 5.0 µg of alpha-TbOH/kg of fresh liver equivalent). Purified extracts were quantitatively analysed by two methods:
- RIA (radio-immunoassay) using an anti-alpha-TbOH antiserum (limit of detection = 0.7 µg/kg).
- GC-MS (gas chromatography-mass spectrometry) of MO-TMS (methyloxime-trimethylsilyl) derivatives (limit of detection = 0.5 µg/kg).

The results of the certification study demonstrated on one hand the fact that many laboratories still have significant problems with the analysis of samples of liver for Trenbolone. However, at the same time laboratories that do have a validated method operational showed to be able to produce good results.

The certified values obtained for the CRMs are:
BCR-474 (blank) < 0.5 µg/kg
BCR-475 (positive) 7.6 ug/kg, uncertainty 2.2 ug/kg.

Additional information

Authors: GASPAR P, Laboratory of Analysis of Foodstuffs of Animal Origin, Faculty of Veterinary Medicine, University of Liège (BE);VAN VYNCHT G, Laboratory of Analysis of Foodstuffs of Animal Origin, Faculty of Veterinary Medicine, University of Liège (BE);SCIPPO M L, Laboratory of Analysis of Foodstuffs of Animal Origin, Faculty of Veterinary Medicine, University of Liège (BE);MAGHUIN-ROGISTER G, Laboratory of Analysis of Foodstuffs of Animal Origin, Faculty of Veterinary Medicine, University of Liège (BE);KRAMER G N, EC, JRC, Institute for Reference Materials and Measurements, Geel (BE);VAN GINKEL L A, National Institute of Public Health and the Environment (RIVM), Laboratory for Residue Analysis, Bilthoven (NL);DIRSCHERL C, EC, DG for Research, Standards, Measurements and Testing programme Brussels
Bibliographic Reference: EUR 20488 EN (2002), pp.42. Free of charge
Availability: EUR-OP reference: KI-NA-20488-EN-C Available from: Documentation Service of DG Research Fax: +32 2 295 8220 E-mail: research@ec.europa.eu
ISBN: ISBN: 92-894-4384-7
Record Number: 200215679 / Last updated on: 2003-01-09
Category: PUBLICATION
Original language: en
Available languages: en
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