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Abstract

The report describes the preparation and certification of dry-mixed soya powder CRMs (IRMM-410S-0) with different mass fractions of genetically modified Roundup Ready soya powder. The CRMs were produced in 2001 by the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the validation and calibration of methods for the detection of genetically modified food. The Roundup Ready concentration of IRMM-410S was verified with the help of DNA based and protein based detection methods. The CRMs are available in the form of glass bottles containing 1 g of soya powder packed under argon atmosphere. Seeds of non-modified soya (line A1900) and Roundup Ready (event 40-3-2, line AG5602 RR) both produced by Asgrow (Faribault, MO, USA) and delivered by Monsanto (St Louis, MO, USA) were rinsed with demineralised water, additionally drained and dried at 30°C in order to minimise dust contamination from other crops. The materials were prepared by quantitative Turbula and dry-mixing of non-modified soya powder and 100% Roundup Ready GMO soya powder. It is recommended to use samplings not smaller than 100 mg. Bottles should be stored dry and cool (+4°C) and kept in the dark.

Additional information

Authors: TRAPMANN S ET AL, European Commission, Joint Research Centre, Institute for Reference Materialsand Measurements (IRMM), Geel (BE);VAN DEN EEDE G, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);WEIGHARDT F, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);MAZZARA M, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);ANKLAM E, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR 20273 EN (2002), pp.21. Free of charge EUR-OP reference: LA-NA-20273-EN-C
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9843 or +39 033278 9864 Fax: +39 033278 9623 E-mail: jrc-publications-office@ec.europa.eu
ISBN: ISBN: 92-894-3725-1
Record Number: 200315718 / Last updated on: 2003-01-10
Category: PUBLICATION
Original language: en
Available languages: en
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