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Methods for the official control of probiotics used as feed additives, Volumes I-III

Funded under: FP5-GROWTH


Council Directive 70/524/EEC concerning additives in feeds regulates the use of probiotic organisms. At the outset of the project, according to the Official Journal of the European Communities (11.9.96 No C263/3) some eleven genera of bacteria, three yeasts and one mould were approved for temporary use within specified member states. This was in line with the provisions of Council Directive 93/113/EC on the use and marketing of enzymes, microorganisms and their preparations in animal nutrition. At the present time some nineteen bacterial strains plus Saccharomyces cerevisiae (yeast) are authorised but no mould is permitted for probiotic use for animal feed. Additional microorganisms are on provisional lists.

The project aim was to develop and validate methods for enumeration and identification of authorised probiotics at strain level in feeding stuffs. Specifically the project aimed to cover identification and enumeration of authorised Bacillus spp., Lactobacillus spp., Bifidobacterium spp., Pediococcus spp., Enterococcus spp., and yeasts in a range of different animal feeds.

Additional information

Authors: LEUSCHER R, Central Science Laboratory, York (UK);KNEIFEL W, University of Agricultural Sciences, Vienna (AT);VERNOUX J-P, University of Caen, Caen (FR);STANTON C, Teagase, Fermoy (IE);ALDAMITZ P, Gaiker, Zamudio (ES)
Bibliographic Reference: EUR 20873 EN (2002), various paging. Free of charge
Availability: EUR-OP reference: KI-NA-20-873-EN-C Available from: Documentation Service of DG Research Fax: +32 2 295 8220 E-mail:
ISBN: ISBN: 92-894-6249-3
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