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  • The Certification of Reference Materials of Dry-Mixed Maize Powder with different Mass Fractions of NK603 Maize, Certified Reference Materials ERM-BF415

Abstract

This report describes the preparation and certification of dry-mixed maize powder CRMs with different mass fractions of genetically modified NK603 maize powder (Certified Reference Materials ERM-BF415a, ERM-BF415b, ERM-BF415c, ERM-BF415d, ERMBF415e and ERM-BF415f). Reference Material ERM-BF415 was originally certified as IRMM-415. The CRMs were processed in 2003 and certified in 2004 by the European Commission, Directorate General Joint Research Centre, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the quality control and calibration of methods for the detection of genetically modified food. The NK603 concentration of ERM-BF415 was verified with the help of DNA-based detection methods. The CRMs are available in the form of glass bottles containing 1 g of maize powder packed under argon atmosphere. Seeds of non-modified maize (seed variety RX670) and NK603 maize (line DKC 57-40) both produced by Monsanto (St. Louis, MO, USA) were rinsed with demineralised water, additionally drained and dried at 30 °C in order to minimise dust contamination from other crops. After a two step grinding process, the materials were prepared by turbula-mixing and dry-mixing of non-modified maize powder and NK603 maize powder. ERM-BF415 was certified to contain the following NK603 concentrations: ERM-BF415a certified value < 0.4 g GM / kg maize; ERM-BF415b certified value 1.0 ??0.4 g GM / kg maize; ERM-BF415c certified value 4.9 ??0.5 g GM / kg maize; ERM-BF415d certified value 9.8 ??0.7 g GM / kg maize; ERM-BF415e certified value 19.6 ??0.9 g GM / kg maize; ERM-BF415f certified value 49.1 ??1.3 g GM / kg maize. The minimum sample intake recommended is 100 mg.

Additional information

Authors: ZELENY R, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BERNREUTER A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);LINSINGER T, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);KRAMER G N, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);PAUWELS J, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 20865 EN (2003), 22 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9843 or +39 033278 9864 Fax: +39 033278 9623 E-mail: jrc-publications-office@ec.europa.eu
ISBN: ISBN: 92-894-6425-9
Record Number: 200417435 / Last updated on: 2006-04-06
Category: PUBLICATION
Original language: en
Available languages: en