Preparation and certification of the porcine reference materials for contents (mass fractions) of chlortetracycline (CTC) based on HPLC BCR-695 and -696 (pig liver) BCR-697 and -698 (pig muscle) [...]
This report describes the production and certification of chlortetracycline (CTC)-free and CTC-incurred pig tissues (kidney, liver and muscle) as reference materials. High performance liquid chromatography (HPLC) with fluorescence detection of CTC and 4-epi-CTC was used for all tissue analyses. A preliminary study was performed to optimise the production control. In the main study, 500 vials each of CTC-free and CTCincurred liver (BCR-695 and �696), kidney (BCR-697 and �698) and muscle (BCR-706 and �707) were produced. A stability study was performed over 79 weeks testing samples stored at -18, +4, 20 and 37 °C. No measurable degradation occurred over this time period at any of the storage temperatures. Further, homogeneity of the materials was tested, too. Certified values of the �blank materials� were based on the observed detection limit. Certified values for the CTC-positive materials are accompanied by a GUMcompliant uncertainty statement (coverage factor k = 2)  considering uncertainty contributions from the batch characterisation, homogeneity, stability as well as from the common calibrant used in the certification exercise.
Bibliographic Reference: EUR 21062 EN (2004), 45 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: email@example.com or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 2
ISBN: ISBN 92-894-3890-8
Record Number: 200417517 / Last updated on: 2004-06-01
Original language: en
Available languages: en