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  • Preparation and certification of the porcine reference materials for contents (mass fractions) of chlortetracycline (CTC) based on HPLC. BCR-695 and -696, BCR-697 and -698, BCR-706 and -707

Abstract

This report describes the production and certification of chlortetracycline (CTC)-free and CTC incurred pig tissues (kidney, liver and muscle) as reference materials. High performance liquid chromatography (HPLC) with fluorescence detection of CTC and 4-epi-CTC was used for all tissue analyses. A preliminary study was performed to optimise the production control. In the main study, 500 vials each of CTC-free and CTC-incurred liver (BCR-695 and �696), kidney (BCR-697 and - 698) and muscle (BCR-706 and �707) were produced. A stability study was performed over 79 weeks testing samples stored at -18, +4, 20 and 37 °C. No measurable degradation occurred over this time period at any of the storage temperatures. Further, homogeneity of the materials was tested, too. Certified values of the �blank materials� were based on the observed detection limit.

Certified values for the CTC-positive materials are accompanied by a GUM-compliant uncertainty statement (coverage factor k = 2) [1] considering uncertainty contributions from the batch characterisation, homogeneity, stability as well as from the common calibrant used in the certification exercise.

Additional information

Authors: JUHEL-GAUGAIN M, CNEVA -Fougeres, Fougeres (FR);VAN GINKEL L A, National Institute of Public Health and Environment (RIVM), Bilthoven (NL);GAWLIK B M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BERNREUTHER A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);LINSINGER T, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);LAMBERTY A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MCEVOY J D G, The Queen�s University of Belfast, Faculty of Agriculture and Food Science, Belfast (UK)
Bibliographic Reference: EUR 21062 EN (2004), 45 pp. Free of charge
Availability: Available from European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE) Tel: +32 (0)14 571 221 Fax: +32 (0)14 590 406 E-mail: jrc-publications-office@ec.europa.eu
ISBN: ISBN 92-894-3890-8
Record Number: 200417551 / Last updated on: 2004-06-15
Category: PUBLICATION
Original language: en
Available languages: en