Community Research and Development Information Service - CORDIS

Abstract

Clenbuterol is a xenophobic orally active beta-agonist. It has been illegally applied as a repartitioning agent in meat producing animals in the EU. The misuse of this pharmacologically active substance has resulted in several cases of food poisoning, which underlines the necessity of developing proper detection methods and control systems. Two clenbuterol standards in bovine eyes were produced and certified: a negative control material, BCR-673 (bovine eye, clenbuterol free; <0.50mu g/kg) and a positive material, BCR-674 (bovine eye containing clenbuterol -10mu g/kg). Eye liquid content with a strong clenbuterol accumulation and slow elimination is the most sensitive tissue for the detection of clenbuterol misuse. It was initially confirmed in this study that approximately 10mu g/kg clenbuterol in eye matrix is suitable for control of clenbuterol misuse. This report describes the production of BCR-673 and BCR-674 using eyes from 103 German Simmental cattle. Materials were checked for their homogeneity and a 3 year stability study at various storage temperatures (-60 degree Celsius, -20 degree Celsius, +4 degree Celsius, +20 degree Celsius and +37 degree Celsius) was performed. The candidate-CRMs were certified in a certification exercise involving 11 EU laboratories. The certified values are close to the desired target concentration of <0.5mu g/kg and approximately 10mu g/kg originally specified.

Additional information

Authors: PFAFFL M W, Scientific Centre of Food Science, Instutute of Physiology, T.U. of Munich, Freising-Weihenstephan (DE);MEYER H H D, Scientific Centre of Food Science, Instutute of Physiology, T.U. of Munich, Freising-Weihenstephan (DE);GAWLIK B M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BERNREUTHER A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);LAMBERTY A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BENNINK D, European Commission, DG Research, Standards, Measurement and Testing, Brussels (BE)
Bibliographic Reference: EUR 21069 EN (2004), 36 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-publications-office@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-894-7115-8
Record Number: 200417591 / Last updated on: 2004-06-07
Category: PUBLICATION
Original language: en
Available languages: en
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