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  • The certification of the mass fractions of PAH metabolites (1-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenzo[a]pyrene) in two fish bile materials, BCR-720, BCR-721


This report describes the preparation, homogeneity study, stability study and certification of two fish bile materials: BCR-720, sediment-exposed flounder bile and BCR-721, oil-exposed plaice bile. In BCR-720 three metabolites of polycyclic aromatic hydrocarbons (PAHs) are certified: 1-hydroxy phenanthrene, 1-hydroxy pyrene and 3-hydroxy benzo[a]pyrene. 1-hydroxy pyrene is certified in BCR-721. A description of the analytical procedures used in the homogeneity and stability studies as well as in the certification study is given. All relevant data from the homogeneity and stability study and certification measurements are presented. Major conclusions from earlier feasibility studies, the results obtained for 2-hydroxy naphthalene (not certified), and implications for the optimal use of the materials are also discussed.

Additional information

Authors: ARIESE F, Vrije Universiteit Amsterdam, Institute for Environmental Studies, Amsterdam (NL);BEYER J, RF-Rogoland Research, Stavanger (NO);WELLS D, Fisheries Research Services, Marine Laboratory, Aberdeen (GB);MAIER E A, European Commission, DG Research, Standards, Measurement and Testing, Brussels (BE);GAWLIK B M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);LAMBERTY A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 21068 EN (2004), 49 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-894-7114-X
Record Number: 200417592 / Last updated on: 2004-06-07
Original language: en
Available languages: en
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