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  • The certification of reference materials of dry-mixed maize powder with different mass fractions of NK603 maize certified reference materials IRMM-415

Abstract

This report describes the preparation and certification of dry-mixed maize powder CRMs with different mass fractions of genetically modified NK603 maize powder (Certified Reference Materials IRMM-415-0, IRMM-415-1, IRMM-415-2, IRMM-415-3, IRMM-415-4 and IRMM-415-5). The CRMs were processed in 2003 and certified in 2004 by the European Commission, Directorate General Joint Research Centre, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the quality control and calibration of methods for the detection of genetically modified food. The NK603 concentration of IRMM-415 was verified with the help of DNA-based detection methods. The CRMs are available in the form of glass bottles containing 1g of maize powder packed under argon atmosphere. Seeds of non-modified maize (seed variety RX670) and NK603 maize (line DKC 57-40) both produced by Monsanto (St. Louis, MO, USA) were rinsed with demineralised water, additionally drained and dried at 30°C in order to minimize dust contamination from other crops. After a two step grinding process, the materials were prepared by turbula-mixing and dry-mixing of non-modified maize powder and NK603 maize powder. IRMM-415 was certified to contain the following NK603 concentrations: IRMM-415-0 certified value <0.4gGM/kg maize; IRMM-415-1 certified value 1.0+/-0.4gGM / kg maize; IRMM-415-2 certified value 4.9+/-0.5gGM/kg maize; IRMM-415-3 certified value 9.8 ± ?0.7 g GM / kg maize; IRMM-415-4 certified value 19.6+/-0.9gGM/kg maize; IRMM-415-5 certified value 49.1+/-1.3gGM/kg maize. The minimum sample intake recommended is 100mg.

Additional information

Authors: TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONNEELY P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GANCBERG D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GIORIA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);VAN NYEN G, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);EMONS H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 21270 EN (2004), **pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-publications-office@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-894-8063-7
Record Number: 200417683 / Last updated on: 2004-08-18
Category: PUBLICATION
Original language: en
Available languages: en