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  • The Certification of Reference Materials of Dry-Mixed Maize Powder with different Mass Fractions of GA21 Roundup Ready Maize, Certified Reference Materials ERM BF-414

Abstract

This report describes the preparation and certification of dry-mixed maize powder CRMs with different mass fractions of genetically modified GA21 RoundupReady maize powder (Certified reference materials IRMM-414-0, IRMM-414-1, IRMM-414-2, IRMM-414-3, IRMM-414-4 and IRMM-414-5). The CRMs were produced in 2003 by the European Commission Directorate-General Joint Research Centre, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. The CRMs are intended for the quality control and calibration of methods for the detection of genetically modified food. The GA21 concentration of IRMM-414 was verified with the help of DNA-based detection methods.
The CRMs are available in the form of glass bottles containing 1 g of maize powder packed under argon atmosphere. Seeds of non-modified maize (seed variety 'RX670') and GA21 maize (event RX740RR) both produced by Monsanto (St. Louis, MO, USA) were rinsed with demineralised water, additionally drained and dried at 30°C in order to minimise dust contamination from other crops. The materials were prepared by quantitative turbula and dry-mixing of non-modified maize powder and GA21 maize powder. It is recommended to use samplings not smaller than 100 mg.

Additional information

Authors: TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONNEELY P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONTRERAS M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GANCBERG D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GIORIA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);EMONS H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 21203 EN (2005), 16 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-publications-office@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-894-7953-1
Record Number: 200518322 / Last updated on: 2005-09-23
Category: PUBLICATION
Original language: en
Available languages: en
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