Community Research and Development Information Service - CORDIS

Abstract

This report describes the preparation and certification of maize powder CRMs with different mass fractions of genetically modified (GM) 59122 maize powder (Certified Reference Materials ERM-BF424a, ERM-BF424b, ERM-BF424c and ERM-BF424d). The materials were processed and certified in 2006 by the European Commission, Directorate General Joint Research Centre, the Institute for Reference Materials and Measurements (IRMM) in Geel, Belgium. Seeds of non-modified maize and 59122 maize both supplied by Pioneer Hi-Bred International (Johnston, IA, USA) were rinsed with demineralised water, drained and dried at 30 degree Celsius in order to minimise dust contamination from other crops. After a two step grinding process, transforming the seeds into a non-modified maize powder and a 59122 GM maize powder, the mixtures, containing different mass fractions of GM, were gravimetrically prepared by dry-mixing. The CRMs are intended for the quality control and calibration of methods for the detection of genetically modified food. The 59122 mass fractions of ERM-BF424 were verified with the help of DNA-based detection methods. The CRMs are available in glass bottles containing 1 g of maize powder packed under argon atmosphere.

Additional information

Authors: GANCBERG D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BROEDERS S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TEIPEL K, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONNEELY P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONTRERAS M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);HANNES E, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GIORIA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);VAN NYEN M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);EMONS H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 22333 EN (2006), 18 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-79-02755-7
Record Number: 200718795 / Last updated on: 2007-02-21
Category: PUBLICATION
Original language: en
Available languages: en