Community Research and Development Information Service - CORDIS

Abstract

This report describes the production and certification of a reference material of purified genomic DNA (gDNA) of Campylobacter jejuni (NCTC 11351). This CRM (IRMM-448) supports the harmonisation and validation of PCR procedures by their use as taxonomic controls in PCR reactions. The homogeneity and stability (at three different temperatures for up to 4 weeks) of the batch was assessed by monitoring the amplification of the ceuE gene by PCR, by agarose gel electrophoresis of gDNA and determination of the mass of gDNA per vial. The material is stable at 4 and 18 degree Celsius. The identity of the gDNA was confirmed by DNA sequence analysis of the ceuE gene. Each vial contains a certified mass of 71 ng freeze-dried gDNA, with an expanded uncertainty (UCRM) of 39 ng (k = 2).

Additional information

Authors: VAN IWAARDEN P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MEEUS N, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);PHILIPP W J, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 22504 EN (2007), 26 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 92-9-03469-3
Record Number: 200718796 / Last updated on: 2007-02-21
Category: PUBLICATION
Original language: en
Available languages: en