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  • Certification Report, The certification of reference materials of maize seed powder with different mass fractions of the maize event 3272, Certified Reference Materials ERM-BF420

Abstract

This report describes the processing and certification of three maize powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) Event 3272 maize (ERM-BF420a, ERM-BF420b and ERM-BF420c). The CRMs were processed and certified in 2006-2007 by the European Commission, Directorate General Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, Belgium.
Hybrid, hemizygous seeds of Event 3272 maize and of its near-isogenic non-GM counterpart were dried and ground to GM and non-GM base powders in a two-step grinding process. A non-GM pure material and two gravimetric mixtures of non-GM and GM maize powder were prepared by dry-mixing. The certified values of these CRMs were calculated from the gravimetric preparations, taking into account the GM purity of the base materials and their water mass fraction. The certified values were confirmed by event-specific realtime PCR as independent verification method.
The CRMs are intended to be used for quality control or calibration of methods for the quantification of the Event 3272 maize mass fraction in food and feed. The CRMs are available in glass bottles containing 1 g of dried maize powder closed under argon atmosphere. The minimum amount of sample to be used per analysis is 100 mg.
The three CRMs (ERM-BF420a, ERM-BF420b and ERM-BF420c) have been accepted as European Reference Material (ERM) after peer evaluation by the partners of the European Reference Materials consortium.

Additional information

Authors: BROOTHAERTS W, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CHAROUD-GOT J, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONNEELY P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CONTRERAS M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GIORIA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);VAN NYEN M, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);VINCENT S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 22698 EN (2006), 22 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-05219-4
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