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  • Certification of the amount-of-substance fraction of HbA0 versus the sum of all Hb isoforms forming the glycated or non-glycated N-terminal hexapeptide of the beta -chain in haemoglobin [...]

Abstract

Certification of the amount-of-substance fraction of HbA0 versus the sum of all Hb isoforms forming the glycated or non-glycated N-terminal hexapeptide of the beta -chain in haemoglobin isolated from whole blood, IRMM/IFCC-467
This report describes the preparation, homogeneity, stability and certification studies as well as the purity evaluation of highly purified HbA0 (IRMM/IFCC-467) from human blood. HbA0 is defined as the non-glycated haemoglobin, in contrast to HbA1c, defined as the stable adduct from glucose and the N-terminal amino group of the ß-chain of haemoglobin A0 that is beta-N-(1-deoxyfructos-1-yl) haemoglobin [1]. A description of the analytical methods used in each of these studies is included. All relevant data from the homogeneity and stability studies as well as those for the characterization measurements are presented.

Additional information

Authors: MUÑOZ-PIÑEIRO A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);KLEIN C, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 22788 EN (2007), 27 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-06043-4
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