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  • Certification Report: Certification of a MON 810 Maize Reference Material for its DNA copy number ratio Certified Reference Material ERM-BF413d
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Certification Report: Certification of a MON 810 Maize Reference Material for its DNA copy number ratio Certified Reference Material ERM-BF413d

Funded under: FP7-JRC

Abstract

This report describes the certification of the Certified Reference Material ERM-BF413d, composed of conventional and genetically modified MON 810 maize powder, for its DNA copy number ratio.
The genetically modified Zea mays L. line MON 810 (Yieldguard) was genetically engineered to resist the European corn borer (Ostrinia nubilalis) and is commercialised by Monsanto Company. This line was developed by introducing the cryIA(b) gene from the common soil bacterium Bacillus thuringiensis (Bt) into the maize cultivar Hi-II by particle acceleration (biolistic) transformation. The cryIA(b) gene produces the insect control protein CryIA(b), a delta-endotoxin.
ERM-BF413d is part of a set of maize CRMs containing different mass fractions of genetically modified MON 810 maize. The CRM was processed and originally certified for its mass fraction by the European Commission, Directorate General Joint Research Centre, Institute for Reference Materials and Measurements, RM Unit, Geel, Belgium [1].
European Commission Recommendation (EC) No 787/2004 advises to express the content of genetically modified food and feed as the percentage of genetically modified DNA copy numbers in relation to target taxon-specific DNA copy numbers calculated in terms of haploid genomes [2], the so-called DNA copy number ratio. An interlaboratory comparison was, therefore, conducted in 2007 to certify the existing CRM for the DNA copy number ratio. The CRM is intended for quality control of measurements of the MON 810 DNA copy number ratios in genetically modified food and feed, using the plasmidic DNA ERM-AD413 [3] for calibration and the event-specific MON 810 detection method ISO 21570:2005, Annex D2 [4]. ERMAD413 contains a 170 bp fragment of the MON 810 plant/P35S junction [5] and a 351 bp fragment of the maize endogenous high mobility group gene (hmg) [6], specific for MON 810 maize and the maize taxon, respectively.

Additional information

Authors: CHARELS D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);BROEDERS S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GIORIA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);VINCENT S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);SCHIMMEL H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);EMONS H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 23028 EN (2007), 18 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-07463-9
Record Number: 200819626 / Last updated on: 2008-01-21
Category: PUBLICATION
Original language: en
Available languages: en