Community Research and Development Information Service - CORDIS

Abstract

This document provides the first general overview on PCR primers used by Member State control laboratories for the detection, identification and quantification of GMOs. The data has been collected from a survey launched in 2005.
The survey aimed at reviewing the PCR primers used by ENGL members for control purposes and at gathering analytical details on the corresponding PCR procedures. It further aimed to provide a catalogue of DNA sequences as a basis for the future development of plasmid standards.
Participants to the survey were specifically requested to provide information on the genetic targets and if relevant, the GM event for which the primers were designed, to include the sequences of primers and the purpose (qualitative/quantitative and screening/identification analysis), type (i.e. single, multiplex, competitive, double-competitive, simplex real-time or multiplex real-time) and specificity (gene-specificity, construct specificity or event-specificity) of the corresponding PCR assay. They were further requested to provide comments and references, as well as to specify the confidentiality status of data provided.

Additional information

Authors: BONFINI L, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);MOENS W, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);BEN E, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);QUERCI M, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);AYGUN B, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);CORBISIER P, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);MORISSET D, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);ZEL J, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);VAN DEN EEDE G, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR 23059 EN (2007), 59 pp. Free of charge
Availability: Katalogue Number: LB-NA-23059-EN-C The PDF version can be downloaded from: http://bookshop.europa.eu
ISBN: ISBN 978-92-79-08006-7
Record Number: 200819731 / Last updated on: 2008-05-05
Category: PUBLICATION
Original language: en
Available languages: en