Validation of an analytical method to determine the content of sucralose in beverages
An inter-laboratory comparison was carried out to evaluate the performance characteristics of a method for the determination of sucralose in beverages, which was developed at the JRC-IRMM. The method is based on high-performance thin layer chromatography (HPTLC), and reagent-free derivatisation followed by ultraviolet/fluorescence detection. It was tested for the determination of Sucralose (C12H19Cl3O8; (2R,3R,4R,5S,6R)-2-(2R,3S,4S,5S)-2,5-bis(chloromethyl)-3,4-dihydroxyoxolan- 2-yloxy-5-chloro-6-hydroxymethyl)oxane-3,4-diol; CAS No: 56038-13-2) in carbonated and still alcoholic and non-alcoholic beverages at proposed European regulatory limits according to Directive 2003/115/EC. Precise determination of Sucralose levels in some of the matrices for which European legislative limits apply, required a robust and reliable analytical method. HPTLC employing reagent-free derivatisation offered such a reliable but simple, fast, cost-effective and environment friendly method (very limited quantities of organic solvents methanol and acetonitrile were used). Separation of Sucralose was performed by direct application of samples (diluted, degassed and/or filtered, if necessary) on amino-bonded silica gel HPTLC plates without prior cleanup and development with acetonitrile-water. The sweetener was determined after heating of the developed plate to 190°C and quantified both in ultraviolet absorption and fluorescence measurement mode. Beverages spiked with sucralose as well as beverages taken from the market and labelled to contain sucralose, were sent to 14 laboratories in five different countries following IUPAC guidelines. A sample that did not contain measureable amounts of sucralose was spiked at levels of 30.5 mg/L, 100.7 mg/L and 299 mg/L. Recoveries ranged from 104 - 125 % with an average of 112 % for ultraviolet detection and from 98 - 101 % with an average of 100 % for fluorescent detection.
Bibliographic Reference: EUR 23056 EN (2007), 62 pp. Free of charge
Availability: Katalogue Number: LA-NA-23056-EN-C The PDF version can be downloaded from: http://bookshop.europa.eu
Record Number: 200819833 / Last updated on: 2008-05-05
Original language: en
Available languages: en