Community Research and Development Information Service - CORDIS

Abstract

The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF) (see Regulation EC No 1829/2003), has carried out an in-house verification study to assess the performance of two quantitative, event-specific methods for use with the hybrid oilseed rape line (unique identifier ACS-BN 5-8xACS-BNøø3-6) which combines both the Ms8 and Rf3 transformation events. Both methods have been previously fully validated individually on parental lines, to detect and quantify each insert on extracted DNA. The study was conducted according to internationally accepted guidelines (1,2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Bayer CropScience provided the detection methods and the samples (genomic DNA extracted from the wild-type and 100% event Ms8xRf3 hybrid oilseed rape). The JRC prepared the in-house validation samples (calibration samples and blind samples at unknown GM percentage). The results of the in-house verification study were evaluated with reference to ENGL method performance requirements and to the validation results for the individual parental lines.

Additional information

Authors: MAZZARA M, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);GRAZIOLI E, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);SAVINI C, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);VAN DEN EEDE G, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR 22929 EN (2007), 40 pp. Free of charge
Availability: Katalogue Number: LB-NA-22929-EN-C The PDF version can be downloaded from: http://bookshop.europa.eu
ISBN: ISBN 978-92-79-06986-4
Record Number: 200819835 / Last updated on: 2008-05-05
Category: PUBLICATION
Original language: en
Available languages: en
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