Report on the verification of the performance of 59122 and NK603 event-specific methods on the hybrid maize line 59122xNK603 using real-time PCRFunded under: FP7-JRC
The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, has carried out an in-house verification study to assess the performance of two quantitative event-specific methods on the hybrid maize line 59122xNK603 which combines the 59122 and NK603 transformation events. The two methods have been validated individually on single-trait events, to detect and quantify each event in maize samples. This study was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Pioneer Hi-Bred Intl. Inc. provided the detection methods and the control samples (59122xNK603 ground maize flour, conventional ground maize flour and non genetically modified control DNA). The JRC prepared the in-house verification samples (calibration samples and blind samples at different GM percentages). The results of the in-house verification study were evaluated with reference to ENGL method performance requirements (http://gmo-crl.jrc.it/doc/Method%20requirements.pdf) and to the validation results on the individual parental events (http://gmo-crl.jrc.it/statusofdoss.htm). The results of this CRL-GMFF in-house verification studies are made publicly available at http://gmo-crl.jrc.it/.
Bibliographic Reference: EUR 23271 EN (2008), 48 pp. Free of Charge
Availability: http://bookshop.europa.eu/is-bin/INTERSHOP.enfinity/WFS/EU-Bookshop-Site/en_GB/-/EUR/ViewPublication-Start?PublicationKey=LBNA23271 (Catalogue Number: LB-NA-23271-EN-C)
ISBN: ISBN: 978-92-79-08501-7
Record Number: 200819952 / Last updated on: 2008-07-28
Original language: en
Available languages: en