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FP7

Report on the verification of the performance of 59122, 1507 and NK603 event-specific methods on the hybrid maize line 59122x1507xNK603 using real-time PCR

Funded under: FP7-JRC

Abstract

The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, has carried out an in-house verification study to assess the performance of three quantitative event-specific methods on the hybrid maize line 59122x1507xNK603 which combines the 59122, 1507 and NK603 transformation events. The three methods have been validated individually on single-trait events, to detect and quantify each event in maize samples. This study was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Pioneer Hi-Bred Intl. Inc. provided the detection methods and the control samples (59122x1507xNK603 ground maize flour and conventional ground maize flour). The JRC prepared the in-house verification samples (calibration samples and blind samples at different GM percentages). The results of the in-house verification study were evaluated with reference to ENGL method performance requirements and to the validation results on the individual parental events.

Additional information

Authors: DELOBEL C, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);PRICE S, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);MAZZARA M, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);SAVINI C, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT);VAN DEN EEDE G, European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (IT)
Bibliographic Reference: EUR 23272 EN (2008), 60 pp. Free of charge
Availability: http://bookshop.europa.eu/is-bin/INTERSHOP.enfinity/WFS/EU-Bookshop-Site/en_GB/-/EUR/ViewPublication-Start?PublicationKey=LBNA23272 (Catalogue Number: LB-NA-23272-EN-C)
ISBN: ISBN: 978-92-79-08523-9
Record Number: 200910070 / Last updated on: 2008-11-20
Category: PUBLICATION
Original language: en
Available languages: en