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Certification Report: Certification of reference materials of maize seed powder with different mass fractions of genetically modified 98140 maize, Certified Reference Material ERM-BF427

Funded under: FP7-JRC

Abstract

This report describes the processing and certification of four maize seed powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) DP-9814-6 maize (ERM-BF427a, b, c, d). The materials were processed and certified in 2008 by the European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM, Geel, Belgium), according to the principles of ISO Guide 34.
Heterozygous seeds of GM DP-9814-6 maize and of a comparable non-GM maize variety were washed, dried and ground to GM and non-GM base powders. A non-GM material and three gravimetric mixtures of non-GM and GM maize seed powder (containing respectively 5, 20 and 100 g/kg GM maize) were prepared by dry-mixing. The material was then milled a second time. The certified values of these CRMs were calculated from the gravimetric preparations, taking into account the GM purity of the base materials and their water mass fraction. The certified values were confirmed by event-specific real-time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025).

Additional information

Authors: GANCBERG D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);DE ANDRADE SILVA E, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MAZOUA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MERVEILLIE A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TUMBA M-F, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 23537 EN (2009), 20 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-10174-8
Record Number: 200910166 / Last updated on: 2009-02-26
Category: PUBLICATION
Original language: en
Available languages: en