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FP7

Certification Report: Certification of reference materials of soya seed powder with different mass fractions of genetically modified Roundup Ready soya, Certified Reference Materials ERM-BF410k

Funded under: FP7-JRC

Abstract

This report describes the processing and certification of four soya seed powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) GTS 40-3-2 (Roundup Ready) soya (ERMBF410ak, bk, dk, gk). The materials were processed and certified in 2007/2008 by the European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM, Geel, Belgium), according to the principles of ISO Guide 34.
Homozygous seeds of GM GTS 40-3-2 soya and of a comparable non-GM soya variety were dried and ground to GM and non-GM base powders. A non-GM pure material and three gravimetric mixtures of non-GM and GM soya powder (containing respectively 1.0, 10.0 and 100 g/kg GM soya) were prepared by dry-mixing. The remaining > 500 micro meters particles were then sieved out from each of these powders, milled a second time, and added back to the main fraction. The certified values of these CRMs were calculated from the gravimetric preparations, taking into account the GM purity of the base materials and their water mass fraction. The certified values were confirmed by event specific real-time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025).

Additional information

Authors: TRAPMANN S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);GANCBERG D, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);CORBISIER P, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MAZOUA S, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);MERVEILLIE A, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);TUMBA M F, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE);EMONS H, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel (BE)
Bibliographic Reference: EUR 23504 EN (2008), 25 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: jrc-info@ec.europa.eu or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-09797-3
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