Certification of a reference material with Candida albicans (NCPF 3179) at a target level of 1000 colony forming units per material sphereFunded under: FP7-JRC
This report describes the certification of a reference material (IRMM-354) of Candida albicans. Certified Reference Materials (CRMs) for microbiological analysis are indispensable tools for development and validation of detection methods and for the implementation and support of internal and external quality control in the area of microbiological analysis. Each vial of IRMM-354 contains one material sphere of Candida albicans. The homogeneity and stability (at -20 C and -70 C) of the batch was assessed by counting colony forming units (cfu) per material sphere on nutrient agar (NA) and Oxytetracyclin-Glucose-Yeast Extract (OGYE) agar. The material is stable when stored at a maximum temperature of -20 C for up to 18 months. The batch was characterised by six laboratories to establish a certified value of cfu per material sphere on NA and OGYE agar. The certified value for NA is 917 cfu per material sphere with an expanded uncertainty of 168. The certified value for OGYE agar is 912 cfu per material sphere with an expanded uncertainty of 173. In both cases, a coverage factor k = 2 is used, corresponding to a level of confidence of about 95 %. The authenticity of the material was confirmed by DNA sequence analysis of the coding region for the hwp1 gene.
Bibliographic Reference: EUR 23707 EN (2009), 23 pp. Free of charge
Availability: Available from European Commission, JRC Knowledge Management Unit, Ispra (IT) Tel: +39 033278 9893 Fax: +39 033278 5409 E-mail: email@example.com or Bruxelles (BE) Tel: +32 2 295 76 24 Fax: +32 2 299 63 22
ISBN: ISBN: 978-92-79-11254-6
Record Number: 200910490 / Last updated on: 2009-12-04
Original language: en
Available languages: en