Community Research and Development Information Service - CORDIS


A disposible biochip for low-cost DNA analysis (PolymerMicroSensorFab)

Project ID: BRPR980770
Funded under: FP5-GROWTH


Largely driven by the human genome project, the application of micro-systems technology to the field of molecular detection has resulted in a variety of devices, such as DNA-arrays and biochips. Presently, these devices are extensively used in genetic research, research into diseases and drug discovery. These biochips are typically �high-end�, based on silicon technology and contain between 10.000 and 100.000 discrete sites. At each site a small quantity of synthetic DNA - the probe DNA, some 10�s to 100�s of nucleotides long - is immobilised. Each site carries probe DNA with a different base-pair sequence - the recognition sequence.

The DNA to be investigated, for example from a patient, is prepared by extracting the relevant short pieces and linking them with a fluorescent dye or other marker. This target DNA, in solution, is then flushed over the chip. The target DNA will bind to the probe DNA at those sites where the target base-pair sequence matches the recognition sequence.The bound complex is then detected by optical fluorescense of the marker.

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Record Number: 4885 / Last updated on: 2003-03-03
Category: PRJS