Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

New biocompatible materials for sensors

The research has focused on new materials for in vivo sensors according 2 lines, novel experimental materials and treated materials. For the first line of research some biopolymers of polysaccharidic nature were tested as component materials for in vivo biosensors, with particular attention to their stability and biocompatibility. Some determinations of ammonia, creatinine and cholic acids were attempted in biological fluids, with satisfying results for precision and accuracy, based on enzyme biosensors with potentiometric and amperometric detection. For the second line of research treatments of polyvinyl chloride (PVC) surfaces to achieve thromboresistance by heparinization were performed. This method involves two steps:
impregnating PVC with an organic soluble quaternary ammonium salt, tridodecylmethylammonium chloride (TDMAC), and then drying under vacuum until any trace of organic solvent is removed;
heparinzing using an aqueous methanol solution of heparin sodium salt. The antithrombogenenicity of heparinized PVC is evaluated by measurement of the Activated Partial Thromboblastin Time (APTT). The influence of the amount of organic solvent and of contact time between PVC and TDMAC on the APTT value of heparinized PVC has been studied, and hence the optimum conditions for the preparation of heparinized PVC have been established. The results obtained show that by this method it is possible to heparinize PVC with good reproducibility and excellent haemocompatibility (APTT value tending to infinite).


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