Mid-Term Report Summary - SPINDLEDESIGN (Design Principles of Microtubule Cytoskeleton Architectures during Cell Division)
The microtubule cytoskeleton is crucial for a multitude of essential intracellular processes in eukaryotic cells. The mitotic/meiotic spindle segregates the genetic material during cell division. During interphase the cytoskeleton adopts characteristically different architectures. We have now entered an era in which most of the key players involved in the organisation of the cytoskeleton and their functions have been identified. Nevertheless we are far from a mechanistic understanding at the systems level of the functioning of the spindle during cell division or of the alternative architectures of the cytoskeleton during interphase. Which minimal set of components is required for which distinct morphological feature of the cytoskeleton is a big open question. To address this issue, a major goal of this project is to use biochemical reconstitution approaches combined with fluorescence microscopy imaging to better understand the design principles of the spindle and interphase microtubule cytoskeleton architectures as observed in human cells. So far this project succeeded in the reconstitution of an important regulated microtubule nucleation module that controls the locality of microtubule nucleation during cell division (Roostalu et al., NCB, 2015). Furthermore, we could show how geometrical confinement influences the self-organisation of purified motors and microtubules in cell-sized droplets (Baumann & Surrey, JBC, 2014). We were also able to reconstitute the peculiar localisation of the main minus end directed motor dynein to dynamic microtubule plus ends (Duellberg et al., NCB, 2014). The next goals are more complex reconstitutions of the metaphase, anaphase and interphase microtubule cytoskeleton.
Woods, Heather Joanne (Chief Financial Officer)
Record Number: 183371 / Last updated on: 2016-05-27
Information source: SESAM