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ComplexiTE Informe resumido

Project ID: 321266
Financiado con arreglo a: FP7-IDEAS-ERC
País: Portugal

Mid-Term Report Summary - COMPLEXITE (An integrated multidisciplinary tissue engineering approach combining novel high-throughput screening and advanced methodologies to create complex biomaterials-stem cells constructs)

ComplexiTE ultimate goal is to define a multiparametric methodology that allows the screening of biomaterials, cells and culture conditions to define the ideal ones to obtain a bona fide bone tissue-like structure. Bone tissue formation requires an orquestrated interaction between several cell types such as osteoblasts and endothelial cells. ComplexiTE has been taking advantage of adipose tissue as a source of mesenchymal stem cells with high differentiation and proliferation capacity, as well of specific sub-populations with enhanced differentiation potential into the osteogenic and endothelial lineages. Our findings demonstrate the possibility of using a subpopulation of adipose tissue as a single cell source to create 3D bone tissue-like models, which is a clear step forward in the regeneration of vascularized bone tissue. Moreover, the intrinsic vascularization/angiogenic potential of SVF cells has been explored, by means of setting up specific culture conditions in the absence of extrinsic growth factors and its potential will be screened in the generation of vascularized bone-like substitutes.
In addition to the vascularization issue in the field of tissue engineering and regenerative medicine, very little is known regarding the contribution of the transplanted cells over tissue repair/regeneration. This has been hampered by the lack of appropriate methodologies that allows for cell tracking along in vitro cultures and more importantly after transplantation. Aiming to tackle this issue that will be also critical for the imaging and ultimate selection of the screened systems, methods to engineer cells with reporter genes using non-viral vectors are being developed.
Novel marine origin materials were developed, aiming at creating a library of such type of materials. Innovative methodologies have been also developed in order to produce an array of particles of different marine-origin combinations and concentrations and different cell types. Novel hydrogel particles that mimic some aspects of tissue extracellular matrix and convey crucial information at the molecular level determining cellular crosstalk, adhesion, proliferation and differentiation, to name a few, were produced. Those 3D hydrogel-based constructs have been developed under defined (co)-culture conditions for the screening of the potential of several marine-origin biomaterials for the development of bone tissue engineering constructs. So far, both the osteogenic outcome and the maintenance of the endothelial phenotype in vitro have been considered for the selection of the conditions.
Overall, a range of approaches have been developed, under individual converging research areas, that have already allowed to start using an rather original integrated multidisciplinary tissue engineering approach to set-up novel high-throughput screening methods to characterize complex biomaterials-cell constructs.


Rui Luis Gonçalves dos Reis, (Full Professor and Research Group Director)
Tel.: +351 253 510902
Fax: +351 253 510909
Correo electrónico
Número de registro: 183523 / Última actualización el: 2016-06-06
Fuente de información: SESAM
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