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GUVs-3G Report Summary

Project ID: 332078
Funded under: FP7-PEOPLE
Country: Spain

Periodic Report Summary 1 - GUVS-3G (Smart photo-activable devices based in plasmonic nanoparticles: Microfluidic-assisted engineering of a third generation of lipid vesicles)

GUVs-3G develops and exploits microfluidic technologies to engineer giant unilamellar vesicles (GUVs) with tailored properties. The core of these GUVs is a large aqueous compartment with a volume of a hundred picoliters. Payloads remain efficiently trapped within this core by the GUV membrane. The membrane of these smart GUVs is a composite material designed with tunable characteristics: It consists of a bilayer of amphiphilic molecules and contains nanoparticles embedded within the bilayer. The concentration and distribution of the nanoparticles in the bilayer determine the permeability and deformability of the GUV membrane. Importantly, if the nanoparticles are responsive to any external stimuli, these membrane properties can be tuned on demand, at a certain time and at a certain location, through an external actuation. A prominent example of responsive nanoparticles (NPs) is plasmonic NPs; these can be activated by light, producing localized heating of the GUV membrane and thus changes in membrane permeability or even membrane rupture. This phenomenon can be exploited to controllably release payloads from the GUV core, which finds applications in pharmaceutical and cosmetic industries. Moreover, changes in membrane permeability enhance osmotic-driven flows of solvent across the membrane; this causes changes in size or shape of the GUVs. These changes bear a striking resemblance with those observed in cells; therefore, this smart GUVs can be used as model systems to study the physical mechanisms involved in cellular functions such as fusion, endo- and exocytosis, and motility. Furthermore, these changes also enable the use of these GUVs as micro-actuators in macroscopic nastic materials; these smart materials, which are able to change shape in response to the changes in size and shape of the GUVs embedded within the material, find applications in technology.

The exquisite control over the flow of fluids afforded by microfluidic technologies enables the fabrication of highly monodisperse emulsions, drop-by-drop, at typical rates of several hundreds per second. This technology not only enables the fabrication of single emulsions but also multiple emulsions with controlled dimensions and compositions. Concentric water-in-oil-in-water (w/o/w) double emulsion drops are particularly interesting to encapsulate payloads with high efficiency. Therefore, this project proposes the use of concentric water-in-oil-in-water (w/o/w) double emulsion drops with ultrathin oil shells as templates to form smart GUVs with controlled payload content, controlled membrane composition, controlled number of gold NPs and controlled distribution of these NPs in the GUV membrane. To achieve control over NP distribution, this project proposes the use of asymmetric membranes: Membranes with lateral asymmetries or domains that form through the phase separation of membrane lipids, and membranes with transversal asymmetries due to the asymmetric distribution of lipids in the two leaflets that constitute the bilayer membrane. Moreover, this project studies the impact of the concentration and distribution of NPs on the mechanical properties of the GUV membrane in the presence and absence of the external stimulus, which is light, at the adequate wavelength to excite the phonon modes of the membrane through plasmon-phonon energy transfer. Furthermore, this project proposes the development of a screening method based on GUV activity under irradiation: A change in GUV shape under flow can be used to separate active GUVs form inactive GUVs. This screening method may be applied to screen activity in living cells.

Lipid membranes have typical thicknesses of approximately 5 nm; this limits the size of the NPs that can be embedded in the membrane without membrane destabilization. We find that the GUV membrane ruptures if dodecanothiol surface coated gold NPs with a diameter that exceeds 7 nm are embedded in the membrane. Smaller NPs can be embedded at concentrations up to 0.5 wt.%, without membrane rupture using our microfluidic approach. This type of membranes is highly sensitive to osmotic pressure. We observe a drastic decrease in size of the GUV when exposed to a hypertonic solution. The presence of NPs in the membrane also affects lipid phase separations. For example, membranes that contain a ternary mixture of lipids that includes a saturated phospholipid, an unsaturated phospholipid and cholesterol phase separate into two single large domains: A liquid disordered and a liquid ordered domain. However, membranes of the same lipid composition that additionally have NPs embedded form smaller domains. Moreover, upon photo-irradiation, NPs tend to be expelled from the lipid membrane through drastic changes in the shape of the vesicles; this ultimately results in domain coalescence. After this coalescence process, the GUV phase separation resemble to that of a NP-free membrane.

To further modify the mechanical and dynamical properties of these GUVs, we develop a microfluidic approach for the production of asymmetric vesicles. Using water-in-oil-in-oil-in-water triple emulsion drops as templates for GUVs, we produce GUVs with two compositionally different leaflets. The degree of asymmetry that we get is limited by the diffusion of the lipids in the two oil phases and therefore is of approximately 70%. This is the first single-step approach that enables a continuous production of GUVs at typical rates of several hundreds per second. In addition, we have implemented these microfluidic approaches to GUV production in PDMS chips, which enables scaling up their production; this is a requirement in any industrial application.

By the end of the project, we therefore expect to deliver a quantitative description of the mechanical and dynamical properties of GUVs with NPs embedded in the membrane. Furthermore, we expect to use the activity of these GUVs as a screening method. GUVs with different compositions and asymmetry, containing NPs embedded in the membrane, that flow within a microfluidic channel can change in size and shape under photo-irradiation; these changes can be detected using a contour detection algorithm and used as an input to the computer that can activate an electrode, and sort active GUVs in a different channel than those unresponsive to light.

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Maribel Rodríguez Villa, (Administrative officer)
Tel.: +34913946376
Fax: +34913946382


Life Sciences
Record Number: 187473 / Last updated on: 2016-08-19