Community Research and Development Information Service - CORDIS

Periodic Report Summary 1 - LIVINGLASER (A laser made entirely of living cells and materials derived from living organisms)

Matjaž Humar
Condensed Matter Department
J. Stefan Institute
Jamova 39
SI-1000 Ljubljana

Report for “LIVINGLASER” (627274) FP7-PEOPLE-2013-IOF
A laser made entirely of living cells and materials derived from living organisms

The proposed goal of LIVINGLASER was to make a laser composed entirely of living cells and materials derived from living organisms. By using cells producing green fluorescent protein a laser was proposed that self-assembles, self-heals, self-reproduces, evolves and adapts to the environment. A number of applications were envisioned including research and medical uses.
These goals were reached entirely and even surpassed. Not only we were able to make living lasers, but we have also showed standalone microlasers completely embedded inside single cells. Further, we have also developed biocompatible optical fibers, which can be used to pump these lasers deep within human body or for a variety of medical applications such as diagnosis and treatments.

Lasers inside live cells

In the last few decades, lasers have become an important part of our lives, with applications ranging from laser pointers and CD players to medical and research uses. Fluorescent dyes have also become commonplace, routinely used in research and diagnostics to identify specific cell and tissue types. Illuminating a fluorescent dye makes it emit light with a distinctive color. The color and intensity are used as a measure, for example, of concentrations of various chemical substances such as DNA and proteins, or to tag cells. The intrinsic disadvantage of fluorescent dyes is that only a few tens of different colors can be distinguished. In a combination of the two technologies, researchers know that if a dye is placed in an optical cavity – a device that confines light, such as two mirrors, for example – they can create a laser.
Taking it all a step even further, our research, described in the journal Nature Photonics, shows we can create a miniature laser that can emit light inside a single live cell. We made our lasers out of solid polystyrene beads ten times smaller than the diameter of a human hair. The beads contain a fluorescent dye and the surface of the bead confines light, creating an optical cavity. We fed these laser beads to live cells in culture, which eat the lasers within a few hours. After that, we can operate the lasers by illuminating them with external light without any harm to the cells. Then we capture the light emitted from the cells via a spectrometer and analyze the spectrum. The lasers can act as very sensitive sensors, enabling us to better understand cellular processes. For example, we measured the change in the refractive index – the way light travels through the cell – while varying the concentration of salt in the medium surrounding the cells. The refractive index is directly related to the concentration of chemical constituents within the cells, such as DNA, proteins and lipids.
Further, lasers can be used for cell tagging. Each laser within a cell emits light with a slightly different fingerprint that can be easily detected and used as a bar code to tag the cell. Since a laser has a very narrow spectral emission, a huge number of unique bar codes can be produced, something that was impossible before. With careful laser design, up to a trillion cells (1,000,000,000,000) could be uniquely tagged. That’s comparable to the total number of cells in the human body. So in principle, it could be possible to individually tag and track every single cell in the human body. This is a huge leap from cell-tagging methods demonstrated until now, which can tag at most a few hundred cells.
Instead of a solid bead, we have also used a droplet of oil as a laser inside cells. Using a micro pipette, we injected a tiny drop of oil containing fluorescent dyes into a cell. In contrast to the solid bead, forces acting inside the cells can deform the droplets. By analyzing the light emitted by a droplet laser, we can measure that deformation and calculate the force acting on the droplet. It’s a way to get a very precise picture of the kinds of mechanical forces exerted within cells by processes such as cellular migration and division.
Finally, we realized that fat cells already contain lipid droplets that can work as natural lasers. They don’t need to eat or be injected with lasers, just supplied with a nontoxic fluorescent dye. That means each of us already has millions of lasers inside our fat tissue that are just waiting to be activated to produce laser light.
Our new cell laser technology will help us understand cellular processes and improve medical diagnosis and therapies. They could eventually provide remote sensing inside the human body without the need for sample collection. Cell lasers also hold promise as a way of deliver laser for therapies, for example, to activate a photosensitive drug at the target to kill microbes or cancerous cells.
The news about first laser was published in numerous news media including Nature, Science, Yahoo News, Fox News, Discovery Channel News, Scientific American, New Scientist, Tech Radar, Beta Boston, Tech Times, Horizon Magazine (European Commission), etc. as well as foreign language news media, for example Wissenschaft Aktuell (Germany) and Le Scienze (Italy). I was interviewed by local (Primorske Novice) and national newspapers (Delo, Finance, Dnevnik). I had three interviews for TV channels (Slovenia 1, POP TV and Kanal A) including national TV broadcasted in main evening news. I also had numerous interviews on radios (Radio Maribor, Radio Koper) including twice on national radio (VAL 202). Readers and viewers selected me by voting as the Slovenian personality of the week and Personality of the month of Primorska region.

Biocompatible optical waveguides

Advances in photonics have stimulated significant progress in diagnostics, surgery and therapeutics, with many techniques now in routine clinical use. However, the finite depth of light penetration, which is typically less than a few mm’s in tissue, is a serious limitation constraining clinical utility. To address this overriding problem, we have developed implantable light-delivery devices made of polymers that are bio-derived or biocompatible, and biodegradable. In contrast to conventional glass or plastic optical fibres, which must be removed from the body soon after use, the biodegradable and biocompatible waveguides may be used for long-term light delivery and need not be removed as they are gradually resorbed by the tissue. As proof of concept, we demonstrate this paradigm-shifting approach for photochemical tissue bonding for wound closure, leading to faster healing and less scarring. The developed fibres have also great potential for biomedical applications, such as in vivo optical sensing and phototherapy.

Related information


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Life Sciences
Record Number: 187596 / Last updated on: 2016-08-22
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