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Improved techniques for NMR spectroscopy

Researchers have designed new experimental procedures to improve the measurement of nuclear magnetic resonance (NMR) spectra.
Improved techniques for NMR spectroscopy
NMR spectroscopy is an important technique in structural biology and in organic and inorganic chemistry research. Current NMR experiments are limited by low sensitivity and resolution, but improvements in methodology and instrumentation are changing that.

The EU-funded RTACQ4PSNMR (Ultrahigh resolution NMR tools for chemistry and structural biology) initiative aimed to improve the sensitivity and resolution of NMR spectroscopy. The researchers worked to design new types of experiments to enhance and simplify spectra.

The project initially set out to improve the resolution of heteronuclear single quantum correlation (HSQC) experiments, which correlate the signals of atoms of different elements (e.g. hydrogen and carbon) that are bonded to one another.

Researchers were able to develop and apply a real-time HSQC experiment for measurements on small and medium-sized proteins, distributing tools to implement such experiments on NMR spectrometers from different manufacturers.

The team went on to develop real-time versions of more complex experiments, demonstrating significant improvements in resolution.

These results should help improve methods for understanding the structures and functions of proteins, and for developing new drugs.

Related information

Keywords

NMR spectroscopy, nuclear magnetic resonance, structural biology, organic chemistry, heteronuclear single quantum correlation
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