Community Research and Development Information Service - CORDIS

FP7

GLORI Report Summary

Project ID: 292259
Funded under: FP7-PEOPLE
Country: Hungary

Final Report Summary - GLORI (Global analysis of R-loop structures (RNA-DNA hybrids) by advanced microscopic and genetic approaches)

Chromosomal breaks are usually associated with pathological disorders but a growing body of evidence point towards their role in a number of physiological processes, as meiotic recombination or class switch recombination. The main goal of this project was three-fold: first, we aimed to find associations between the occurrence of certain histone modifications / histone modifying enzymes, non-B DNA structures (e.g. RNA-DNA hybrids) and DNA damage formation (as DNA double-strand breaks (DSBs) and single-strand breaks (nicks). Second, we wished to understand how the molecular components of DNA breakage - associated or no with RNA-DNA hybrid formation - is embedded with higher-order chromatin architecture. Third, we wished to characterize protein factors that are involved in sensing, forming, stabilizing, and eliminating scheduled and unscheduled R-loop structures. Our investigations were performed in three model organisms, from S. cerevisiae to human cell lines, upon various metabolic settings and in mitotic and meiotic growth conditions.

Related information

Documents and Publications

Contact

Gabor Szabo, (Head of Cell Biology Division)
Tel.: +36412623
E-mail
Record Number: 196956 / Last updated on: 2017-04-07