Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Design and Simulation of Integrated Biochemical Processes

A methodology for the integrated design of biochemical processes involving initial flowsheet generation using properties based synthesis techniques. This is followed by simulation using a newly developed simulation program for biochemical processes. The evaluation of the best design is done either on technical feasibility or on an environmental criterion based on the use of the area involved in the life cycle of the process.

The methodology has been validated on an industrial scale process for bovine somatotropin (BST) production. The simulation program has also been validated on a process for the production of alpha-amylase. The project involved model development and experimental validation of the models for BST production in E.coli, proteinase A production in yeast and alpha-amylase production in Aspergillus oryzae. These three systems were used for initial verification of the feasibility of the approach prior to more comprehensive validation. A model for chromatographic separations has also been derived and validated with experimental results for alpha-amylase which was used as a model system.

The project has generated a software prototype for biochemical process simulation. It has also generated a methodology for process synthesis although further work is required to integrate the computational aspects of the two. An environmental evaluation procedure for biochemical processes has been developed and implemented. New models for three fermentation systems have been derived and validated. The project also generated a new methodology for rapid collection of the key data for any new chromatographic system.