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ARABIDOPSIS THALIANA BINARY COSMID LIBRARY

The JIC-BC cosmid library of Arabidopsis thaliana (ecotype Columbia) was developed by Dr Ian Bancroft and colleagues at the John Innes Centre, Norwich, UK. The library is constructed using pCLD04541 vector (Bancroft, et al. Weeds World 4(ii) 1-9, 1997) which allows direct use of inserts for Agrobacterium-mediated plant transformation. The Columbia ecotype is the same as that used in the public Arabidopsis genome sequencing programme. The E.coli strain used is a tetracycline sensitive derivative of the Sure strain. Selectable marker are tetracycline for selection in E.coli and Agrobacterium, and kanamycin for selection in transgenic plants. The library is arrayed in 192 high density (384 well) microtitre plates and provides on average 5-fold redundant coverage of the Arabidopsis thaliana genome. The average insert size is 17kb.

Reported by

Plant Bioscience Limited
Norwich Research Park
NR4 7UH Norwich
United Kingdom
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