Wspólnotowy Serwis Informacyjny Badan i Rozwoju - CORDIS

Reference linkage map for forest species in Pinus pinaster and reduced satellite maps in four different conifer species

A High density, reference linkage map for Conifer species has been developed. It contains 1697 AFLP, SSR and EST markers (141 markers per linkage group). This map correponds to a Pinus pinaster population descending from the cross 0024 Landes x C803 Corsica. Total genome length of the integrated map is around 2000 cM. Our linkage map represents actually the map with the highest marker density in forest species.

Furthermore, linkage maps in six different conifer populations are available (2 populations of Pinus radiata, 2 populations of P. sylvestris, one population of Pseudotsuga menziesii and Picea abies). The individual parental and integrated linkage maps show a varying degree of saturation. They contain between 11 and 17 linkage groups and between 54 (Pseudotsuga, parental map) and over 750 markers (Picea, intgrated map). In accordance the map lengths vary between 500 and 2000 cM.

Maps are partially aligned with other published maps based on co-dominant EST and SSR. 9 of the 12 LGs of the reference map could be assigned to published P. taeda linkage groups as well as to 7 Pinus elliottii, 7 P. pinaster (INRA) and 6 Pinus radiata (New Zealand) linkage groups. Moreover, several linkage groups of the P. radiata partner populations could be associated with linkage groups of the reference map and other maps.

Details of maps, markers and alignments including AFLP profiles can be viewed at http://www.neiker.net/UHDfor.

Results are of basic scientific character, but could be applied for marker assisted breeding and enhanced or comparative genomic research. The scientific team of this project can contribute expertise, materials and project results for collaboration in the frame of R&D projects, for providing external commercial services or for technology transfer to companies or start-ups of the Biotech sector.

Potential useful applications of these project results:
- Development of molecular markers and other tools for marker assisted breeding and selection.

- Integration of additional markers and QTLs from external partners into the reference and sattelite maps.

- Construction of sattelite maps in other genetic backgrounds of the same or related species.

- Identification, characterization and mapping of cDNAs involved in response reactions to biotic and abiotic stresses or involved in wood quality.

- Enhancement of the existing reference map by linking a Physical Map and a Transcriptome map, which would allow the isolation and characterization of any gene of interest in the future.

Reported by

NEIKER
Apartado 46
01080 Vitoria
Spain
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