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FP5

FPPARS Report Summary

Project ID: Q5RS-2000-30360
Funded under: FP5-LIFE QUALITY
Country: Spain

Quantitative PCR protocols for tissue expression of PPAR genes in fish

Quantitative real-time PCR (Q-PCR) protocols have been developed and have been successfully used to determine PPAR isotype expression in tissues of sea bream as well as in primary hepatocytes and adipocytes from the same species. The method is based on the amplification of a short region of the cDNA of each of the three sea bream PPAR isotypes using specific primers and specific Taq-man fluorescent probes. As internal standard (reference gene) the sea brean alpha-tubulin has been used.

The protocol results in great sensitivity and accuracy in determining PPAR expression and presents the potential of numerous applications in various aspects of PPAR biology in sea bream. With appropriate adaptations (species-specific primers), this protocol can be used in the determination of PPAR expression in virtually any fish species.

Contact

Jose M. BAUTISTA, (Professor)
Tel.: +34-91-3943827
Fax: +34-91-3943824
E-mail
Record Number: 34037 / Last updated on: 2004-10-14
Information source: e-TIP
Collaboration sought: Further research or development support
Stage of development: Scientific and/or technical knowledge (basic research)
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