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FP5

FPPARS Report Summary

Project ID: Q5RS-2000-30360
Funded under: FP5-LIFE QUALITY
Country: Greece

Sea bream and Atlantic salmon PPAR target genes

Complete or partial cDNAs encoding fatty acid metabolising enzymes have been identified in both sea bream and Atlantic salmon. Specifically, from sea bream a complete cDNA for carnitine palmitoyltransferase 1 (CPT1) and a partial cDNA for glutathione S-transferase A (GSTA) have been isolated. From Atlantic salmon, a partial cDNA for CPT1 has been identified. These cDNAs along with those encoding a fatty acyl elongase and a fatty acyl desaturase (delta 5/6) from sea bream and Atlantic salmon and the GSTA from plaice, that have been previously described, constitute a significant number of potential PPAR-target genes and thus can be used as markers of PPAR-regulated fatty acid metabolism. Important is to note the identification of a partial cDNA encoding the acyl-CoA oxidase from sea bass.

Additional cDNAs that have been identified in the frame of this project, although unrelated to fatty acid metabolism, include the comple coding sequence of alpha-amylase from sea bream and sea bass, a partial cDNA for alpha-tubulin from sea bream, sea bass, and Atlantic salmon, a partial cDNA for beta-actin from sea bream and sea bass, and a partial cDNA for the Atlantic salmon glyceraldehyde phosphate dehydrogenase (GAPDH).

As most of these cDNAs have not been previously reported in the above species, they provide the opportunity for a more thorough examination of their regulation in response to nutritional stimuli or other factors that could affect their expression.

Contact

Grigorios KREY, (Associate Researcher)
Tel.: +30-25940-29035
Fax: +30-25940-22222
E-mail
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