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Development of a fed-batch culture: comparison between control and bcl-2 transfected cell lines

An adaptive, model based process control for fed-batch suspension cultures has been successfully developed and validated for the model cell lines NS0 6A1 (100)3 and NS0 6A1 bcl-2. This control was used for the enhanced fed-batch cultivation of these cell lines. Besides, it served for more detailed process evaluation.

The adaptive, model-based process control was successfully transferred from fed-batch control to the control of the dialysis fed-batch process. In addition, performed time-space yield considerations led to a deeper understanding of the processes. Comparison to hybridoma cell cultivations using the process control showed, how exact the control strategy reacts to different cell lines and culture conditions. Again, the improvement of the bcl-2 over-expression can be clearly seen in the prolonged growth phase and a higher maximum cell density resulting in a higher antibody production.

The results have already been published:
Frahm, B., Lane, P., Märkl, H., Pörtner, R. (2003): Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis, Bioprocess and Biosystems Engineering 26, 1-10 The fed-batch process control is also presented in a published book.: Frahm, B. (2003): Adaptive, modellgestützte Prozessführung von Suspensionskulturen tierischer Zellen, ISBN 3-8334-0111-7.

Reported by

TUHH-Technologie GmbH
Denickestrasse 15
21071 Hamburg
Germany
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