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Improved secretion

Gram-positive bacteria of the species Bacillus are well known for the high levels of secretion of several of their homologous proteins. Attempts to secrete heterologous proteins at high levels have, however, often met with little success. An important result from this project was that mutations in the dlt operon, responsible for the alanylation of cell wall teichoic acids, resulted in a significant increase in the production of a number of secretory proteins, including Bacillus amylases, and the protective antigen (PA) of Bacillus anthracis. This finding holds promise for the improved secretion of other (heterologous) proteins. The results have been published in the international scientific literature and a patent was filed.

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Reported by

Newcastle University
Cell and Molecular Biosciences
NE2 4HH Newcastle upon tyne
United Kingdom
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