Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Method for species-specific detection and identification of Fusarium species in cereals

The result is a set of PCR methods to detect the major Fusarium species responsible for causing head blight of small grain cereals and contaminating grain with mycotoxins. The species include F. sporotrichioides, F. langsethiae, F. armeniacum, F. acuminatum and F equiseti.

The method has been developed to permit detection of these species, within cereal plant tissues and other matrices, using a single PCR protocol. The protocol is identical to a previously developed method to detect additional Fusarium species, F. culmorum, F. graminearum, F. avenaceum and F. poae. All the significant Fusarium pathogens of cereals occurring in Europe can be detected with this highly sensitive, specific method using a single ‘universal’ protocol.

The method has revealed the presence of F. langsethiae across Europe whereas it had previously only been identified from Scandinavian samples. The method will be published within peer-reviewed scientific journals. Licence agreements are foreseen with laboratories both within and outside Europe.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

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Reported by

JOHN INNES CENTRE
Norwich Research Park, Colney
NORWICH
United Kingdom
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