Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

DETOX-FUNGI Résumé de rapport

Project ID: QLK1-CT-1999-01380
Financé au titre de: FP5-LIFE QUALITY
Pays: Norway

Multiplex array method for identification of present Fusarium species in cereal matrices

The result is a modular DNA array system for multiplex detection of Fusarium species. The system can be expanded to include additional taxa, including non-Fusarium species.

The system is built on technology owned by Eppendorf Array Technology (EAT), and is developed in close collaboration with EAT and the University of Namur, Belgium. Specific hybridisation probes are developed on the basis of unique sequence motifs identified from character based phylogenetic analyses of one or more genes. The gene(s) can be PCR amplified with "universal" primers, and the resulting population of amplification products is analysed qualitatively by array hybridisation.

Diagnostic probes will be the intellectual property of the developers, and the EAT technology will be used to provide cheap and affordable arrays and readers for diagnostics. The result will provide a fast, reliable and affordable alternative to conventional microbiological testing (culturing, microscopy) and qualitative PCR (specific primers, gel electrophoresis) and may be particularly useful to screen agricultural crops for presence of potential pathogens and toxigenic fungi. The system is likely to be further developed into a (semi)quantitative system.

The system is under expansion and optimisation and will be subjected to large scale validation prior to commercialisation. Licence agreements are foreseen with laboratories in- and outside Europe.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

Contact

Arne HOLST-JENSEN, (Head of molecular biology unit)
Tél.: +47-23-216243
Fax: +47-23-216202
E-mail