Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

Method for monitoring expression of ochratoxigenic genes in Penicillia

A Real Time RT PCR system has been set up against a putative polyketide synthase gene involved in ochratoxin biosynthesis of P. nordicum. It was possible to monitor and quantify the expression of this gene in vitro and in a food matrix like wheat. Expression of this gene could be detected about 36 h before the first ochratoxin could be determined analytically.

The system is very specific and can be used to monitor the induction of an ochratoxin biosynthetic gene under relevant environmental conditions. The system may be helpful to identify critical control points (CCP´s) in HACCP concept aginst P. nordicum.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

Reported by

Federal Research Centre for Nutrition
Haid-und-Neustr.9
76131 Karlsruhe
Germany
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