Community Research and Development Information Service - CORDIS

Method for identification and quantification of presence of T-2/HT-2 toxin producing Fusarium species in cereal matrices

The result is a real-time PCR method for quantification of DNA derived from the T-2/HT-2 producing Fusarium species F. langsethiae and F. sporotrichioides. The method determines a relative ratio between cereal derived DNA and DNA from the two fungal species.

The method can be used to determine the infection level in a wide range of cereal matrices, and may prove particularly useful for the farmer at pre-harvest, and for food and feed manufacturers and retailers, and competent authorities at post-harvest.

The method is much faster and several times more sensitive than conventional microbiological methods, and a high degree of correlation has been observed between infection levels (determined with the method) and toxin levels (determined by chemical analyses).

The method will be published in a peer-reviewed scientific journal. A patent covering the specific oligonucleotides (primers and probes) is foreseen. Manufacturing and licence agreements are foreseen with laboratories in- and outside Europe.

More information on the DETOX-FUNGI project can be found at http://detox.ba.cnr.it.

Related information

Contact

Arne HOLST-JENSEN, (Head of molecular biology unit)
Tel.: +47-23-216243
Fax: +47-23-216202
E-mail
Follow us on: RSS Facebook Twitter YouTube Managed by the EU Publications Office Top