Community Research and Development Information Service - CORDIS

Method for the species specific identification and detection of Aspergillus ochraceus using PCR based on a pair of SCAR primers

The method is based on a pair of PCR primers which was designed upon sequence information of a fragment specifically encountered in AFLP patterns prepared from genomic DNA of Aspergillus ochraceus.

The fragment was sequenced and a pair of primers was designed. PCR using the said primer pair (OCA-V/OCA-R) yielded a 258bp product with genomic DNA isolated from various strains of A. ochraceus but not with DNA isolated from other closely related fungal species.

The system was optimised to result in highest sensitivity and specificity. It may be commercially exploited in applications where this ochratoxin a producing fungus can be detected in pure cultrures and in contaminated samples, e.g. green coffee.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

Reported by

Technische Universität München, Lehrstuhl für Technische Mikrobiologie
Weihenstephaner Steig 16
85350 Freising
Germany
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