Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Method for species-specific detection and identification of Fusarium proliferatum and F. oxysporum from asparagus plants

Fusarium proliferatum and Fusarium oxysporum are the causal agents of a destructive disease of asparagus called Fusarium crown and root rot. F. proliferatum from asparagus produces fumonisin B1 and B2, which have been detected as natural contaminants in infected asparagus plants.

Polymerase chain reaction (PCR) assays were developed for the rapid identification of F. proliferatum and F. oxysporum in asparagus plants. The primer pairs are based on calmodulin gene sequences. The PCR products from F. proliferatum and F. oxysporum were 526 and 534bp long, respectively.

The assays were successfully applied to identify both species from the vegetative part of the plants.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

Reported by

CNR
Via Amendola 122/O
70126 Bari
Italy
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