Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS


DETOX-FUNGI Informe resumido

Project ID: QLK1-CT-1999-01380
Financiado con arreglo a: FP5-LIFE QUALITY
País: Germany

Method and kit for rapid preparation of fungal DNA from pure plate cultures for PCR analysis

The method uses a specific lysis buffer and commercially available DNA extraction columns to prepare genomic DNA from agar plugs cut from a living fungal culture. DNA is pure enough to be directly used as template in PCR. Time needed for DNA extraction is less than 10 min per sample.

The method described was demonstrated to be useful in connection with method 9092 for identification of pure cultures of Aspergillus ochraceus. The method may be useful in several similar applications when incorporated into a PCR assay system for fungal organisms.

More information on the DETOX-FUNGI project can be found at:


Ludwig NIESSEN, (Head of fungal molecular biology unit)
Tel.: +49-816-1715496
Fax: +49-816-1713327
Correo electrónico
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