Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Method and kit for rapid preparation of fungal DNA from pure plate cultures for PCR analysis

The method uses a specific lysis buffer and commercially available DNA extraction columns to prepare genomic DNA from agar plugs cut from a living fungal culture. DNA is pure enough to be directly used as template in PCR. Time needed for DNA extraction is less than 10 min per sample.

The method described was demonstrated to be useful in connection with method 9092 for identification of pure cultures of Aspergillus ochraceus. The method may be useful in several similar applications when incorporated into a PCR assay system for fungal organisms.

More information on the DETOX-FUNGI project can be found at: http://detox.ba.cnr.it.

Reported by

Technische Universität München, Lehrstuhl für Technische Mikrobiologie
Welhenstephaner Steig 16
85350 Freising
Germany
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