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Comparison of different routes of vaccination for eliciting antibody responses in the human stomach and duodenum

In this study we have compared different routes of vaccination for eliciting antibody responses in the human stomach and duodenum. H. pylori infected individuals were immunized either nasally or rectally with a model antigen (cholera toxin B subunit) and immune responses after these routes were compared with responses after oral or intrajejunal vaccination.

Specific antibody levels in serum as well as specific antibodies and antibody-secreting cells in biopsies from antrum and duodenum were determined by ELISA and ELISPOT-methods. In contrast to oral vaccination, neither nasal nor rectal vaccination induce significant increases in specific ASC either in antrum or duodenum. Furthermore, when analysing the antibody levels in saponin extracted biopsies, intrajejunal vaccination was superior to both nasal and rectal vaccination in inducing antigen-specific IgA.

We conclude that oral vaccination is the optimal route for induction of antigen-specific responses in the stomach and duodenum in humans while nasal or rectal vaccination are relatively ineffective for this purpose.

More information on the MUCIMM project can be found at: http://www.altaweb.it/mucimm/.

Reported by

University of Göteborg
Guldhedsgatan 10
41346 Göteborg
Sweden
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